This is not a full Reaction Module. 3. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Leak-free, modular, and autoclavable template. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Screen and filter antibodies and sera. Leak-free, modular, and autoclavable template. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Homogenates of liver tissues were prepared according to the manufacturer's protocols (Roche Applied Diagnostic GmbH, Mannheim, Germany). Autoclave Software 1. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. The Bio-Dot microfiltration apparatus can be used for any application requiring rapid immobilization and screening of unfractionated or purified proteins, nucleic acids, or macromolecular complexes on membranes, such as nitrocellulose or Zeta-Probe ® membrane. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Features of the Bio-Dot Microfiltration System: Resistance to 100% ethanol, strong acid, and NaOH; Autoclavability; Patented sealing gasket to eliminate lateral leakage Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket Bio-Dot Apparatus #1706545 | Bio-Rad Skip to main contentReproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Conversion of the Bio-Dot SF apparatus to the Bio-Dot blotting apparatus is accomplished by purchasing the Bio-Dot module, which provides the 96-well sample template. Bio-Dot microfiltration system. office for more information on Bio-Rad blot-ting equipment and reagents. Leak-free, modular, and autoclavable template. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle,. The 96-well Bio-Dot and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Leak-free, modular, and autoclavable template. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. Leak-free, modular, and autoclavable template. Many experimental protocols can be accommodated by using interchangeable templates. Bio-Dot® and Bio. The Bio-Dot® Microfiltration Apparatus (Bio-Rad) is made of polysulfone with a silicone gasket, designed for the application of samples to a membrane using vacuum. Bioz Stars score: 86/100, based on 1 PubMed citations. Leak-free, modular, and autoclavable template. Screen and filter antibodies and sera. The membrane with bound antigen is then incubated with first antibody, specific for the antigen to be detected. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Screen and filter antibodies and sera. This protocol describes the preparation of protein fractions from brain regions or neural cell cultures for the high-throughput quantification of target proteins using a dot. The Bio-Dot Microfiltration System is available as a complete. Leak-free, modular, and autoclavable template. 添加到热点列表. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. and concentrated alkali or acid solutions. 170-6543. Leak-free, modular, and autoclavable template. 00. Leak-free, modular, and autoclavable template. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. 1 Autoclaving The Tygon. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Slot blotting microfiltration device, includes 48-well slot-format sample template, vacuum manifold base, gasket support plate, gasket, filter paper Product Specs; Company Info;. Filter retardation assay is a quick and sensitive method that detects and quantifies protein aggregates formed in vivo and in vitro, including polyglutamine (Scherzinger et al. Both sample templates are interchangeable and use the same microfiltration manifold. Price from $9. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Leak-free, modular, and autoclavable template. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Many experimental protocols can be accommodated by using interchangeable templates. In short, total protein (25 μl per well of a 96 well plate) was transferred onto a nitrocellulose membrane, blocked for 2 h with 5% nonfat milk in TBST (Tris-buffered saline, 0. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. Login | Register. This Bio-Rad iCycler 96 Well Reaction Module Lid is used and in excellent condition. Screen and filter antibodies and sera. 0. Features of the Bio-Dot Microfiltration System: Resistance to 100% ethanol, strong acid, and NaOH; Autoclavability; Patented sealing gasket to eliminate lateral leakageReproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. 概述. Screen and filter antibodies and sera. Leak-free, modular, and autoclavable template. Many experimental protocols can be accommodated by using interchangeable templates. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. PAGE 6 Section 2 Special Handling Features The Bio-Dot apparatus withstands autoclave temperatures for sterilization, as well as cleaning with alcohols, acids, and base solutions. Screen and filter antibodies and sera. ZERO BIAS - scores, article reviews, protocol conditions and moreMicrofiltration blotting— the Immun-Blot assay can easily be adapted for use in the Bio-Dot ® and Bio-Dot SF apparatus. Membrane size 12 x 9 cm sheet. Leak-free, modular, and autoclavable template. Leak-free, modular, and autoclavable template. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Screen and filter antibodies and sera. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Leak-free, modular, and autoclavable template. Overall size 13 x 15 x 6 cm. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. This. ZERO BIAS - scores, article reviews, protocol conditions and moreMicrofiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Descripción. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Many experimental protocols can be accommodated by using interchangeable templates. Proteins were detected using anti-PAR4 (sc-1666 HRP; Santa Cruz Biotechnology) and a Western Lumi-Light sc-2048 (Santa Cruz Biotechnology) transfer. Screen and filter antibodies and sera. Screen and filter antibodies and sera. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Screen and filter antibodies and sera. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasket Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. The Bio-Dot® Microfiltration Apparatus (Bio-Rad) is made of polysulfone with a silicone gasket, designed for the application of samples to a membrane using. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Features of the Bio-Dot Microfiltration System: Resistance to 100% ethanol, strong acid, and NaOH; Autoclavability; Patented sealing gasket to eliminate lateral leakagePlace a pre-wetted membrane in the 96-well Bio-Dot® Microfiltration Apparatus (Bio-Rad) according to the manufacturer’s instructions. Supernatants were collected, and interleukins (IL-1β. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. Rehydrate membrane with ~200ml 10X SSC per well. Description. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Transfer the supernatants (soluble fractions) in a 96-well microplate at 4 °C. Configuration: • Sample Template • Sealing Gasket • Vacuum Manifold • Tubing and Flow Valve The Bio-dot® Microfiltration Apparatus is in a 96 well-plate format, allowing analysis of many small samples at once. Fuji Super RX-N 13x18 film (Fujifilm, catalog number: RX1318) 13. Screen and filter antibodies and sera. Dot blot analyses (Bio-Dot® Microfiltration Apparatus; Bio-Rad Laboratories Inc. A microfiltration apparatus was used to define wells over each sample detection region (Bio-Dot ® Microfiltration apparatus from Bio-Rad, Hercules, CA, USA). Conversion of the Bio-Dot SF apparatus to the Bio-Dot blotting apparatus is accomplished by purchasing the Bio-Dot module, which provides the 96-well sample template. Fo r t e c h n i c a l s e r v i c e c a l l yo u r l o c a l B i o - R a d o f f i c e o r i n t h e U. Many experimental protocols can be accommodated by using interchangeable templates. 5 Section 4 Electrophoretic Blotting. Leak-free, modular, and autoclavable template. Soak membrane and filter papers in TBS. 99. Leak-free, modular, and autoclavable template. Screen and filter antibodies and sera. Bio-Rad 96 well dot blot apparatus 96 Well Dot Blot Apparatus, supplied by Bio-Rad, used in various techniques. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket Bio-Dot Apparatus #1706545 | Bio-Rad Skip to main content Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Screen and filter antibodies and sera. Conversion of the Bio-Dot SF apparatus to the Bio-Dot blotting apparatus is accomplished by purchasing the Bio-Dot module, which provides the 96-well sample template. Leak-free, modular, and autoclavable template. Leak-free, modular, and autoclavable template. Because non-fat dry milk cannotBio-Rad bio rad dot blot sf microfiltration apparatus Bio Rad Dot Blot Sf Microfiltration Apparatus, supplied by Bio-Rad, used in various techniques. Leak-free, modular, and autoclavable template. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. Bio-Rad 96-Well Bio-Dot Microfiltratio n Apparatus, 1706545, Blotting DeviceMicro. 40–60 µg of secretome proteins in 100 µL PBS were dot blotted onto nitrocellulose membranes using the Bio-Dot 96-Well Microfiltration. Leak-free, modular, and autoclavable template. Leak-free, modular, and autoclavable template. Description. Instruction Manual, Bio-Dot Microfiltration Apparatus, Rev DBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Leak-free, modular, and autoclavable template. Chemical compatibility The Bio-Dot SF apparatus can be used with 100% alcohol solutions. Many experimental protocols can be accommodated by using interchangeable templates. Sequi-Blot PVDF membranes (Bio-Rad) were cut to size, wet with MeOH and equilibrated in TE buffer, then assembled into a 96-well Bio-Dot microfiltration apparatus (Bio-Rad). Leak-free, modular, and autoclavable template. Each well was washed. . Leak-free, modular, and autoclavable template. Conversion of the Bio-Dot SF apparatus to the Bio-Dot blotting apparatus is accomplished by purchasing the Bio-Dot module, which provides the 96-well sample template. (b). Vacuum pump 10. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Bio-Rad Bio-Dot 96-Well Microfiltration This Bio-Rad Bio-Dot Apparatus is used and in excellent condition. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketPkg of 1, 96-well sample template, modular unit converts Bio-Dot SF apparatus to Bio-Dot apparatus; for blot transfer microfiltrationOverview. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketBio-Rad Laboratories, Inc. Leak-free, modular, and autoclavable template. Soak the nitrocellulose membrane in 1× PBS for 10 min at room. Adapters are available for the application of either 96 “dots” in a 12x8 array, or as rectangular “slots” in a 6x8 array. Screen and filter antibodies and sera. . when a semi dry apparatus is used with a well optimized gel and transfer system. Reproducibly capture proteins oder nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. 96 Well Dot Blot Hybridization Manifold, supplied by Bio-Rad, used in various techniques. S. Many experimental protocols can be accommodated by using interchangeable templates. Bio Dot Micro Filtration Apparatus, supplied by Bio-Rad, used in various techniques. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketOverview. Description. Leak-free, modular, and autoclavable template. The Bio-Dot SF Microfiltration System is available as. Contact Bio-Rad for pricing and availability. Phosphate-buffered saline (1× PBS): 25 mM Na 2 HPO 4, 150 mM NaCl, pH 8. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto. Condition: New Quantity: 3 available Price: US $499. List Price: Your Price: Log in to see your price. Screen and filter antibodies and sera. Features of the Bio-Dot Microfiltration System: Resistance to 100% ethanol, strong acid, and NaOH; Autoclavability; Patented sealing gasket to eliminate lateral leakage Overview. The Bio-Dot microfiltration apparatus can be used for any application requiring rapid immobilization and screening of unfractionated or purified proteins, nucleic acids, or. The Bio-Dot SF slot format sample template has 48 wells with dimensions of 7 mm x 0. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Many experimental protocols can be accommodated by using interchangeable templates. Bioz Stars score: 96/100, based on 1 PubMed citations. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. Many experimental protocols can be accommodated by using interchangeable templates. Screen and filter antibodies and sera. Vortex (Fisher scientific, model: ZX Wizard vortex, catalog number: 11746744) 12. Features of the Bio-Dot Microfiltration System: Resistance to 100% ethanol, strong acid, and NaOH; Autoclavability; Patented sealing gasket to eliminate lateral leakageBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Leak-free, modular, and autoclavable template. Bioz Stars score: 96/100, based on 1 PubMed citations. Screen and filter antibodies and sera. 1 Autoclaving The Tygon. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. 7. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. The. Screen and filter antibodies and sera. The 96-well Bio-Dot and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. 列表价格: 数量: 添加到报价. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Leak-free, modular, and autoclavable template. Reproducibly capture proteins or core acids in solution onto membranes with Bio-Dot microfiltration systems. Leak-free, modular, and autoclavable template. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketReproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Leak-free, modular, and autoclavable template. The gasket is aligned Bio-Dot microfiltration system. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. 20 °C incubator (LIEBHERR, model: WK 4126) 11. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket Bio-Dot Apparatus #1706545 | Bio-Rad Skip to main content The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Description. Many experimental protocols can be accommodated by using interchangeable templates. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Leak-free, modular, and autoclavable template. . The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Screen and filter antibodies and sera. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. Repeat. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Leak-free, modular, and autoclavable template. Bio-Rad 96 well dot blot apparatus 96 Well Dot Blot Apparatus, supplied by Bio-Rad, used in various techniques. The Bio-Dot Microfiltration System is available as a complete standalone unit (170-3938) or as a modular template without the manifold base ( 170-6547 ). Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Screen and filter antibodies and sera. It is made of polysulfone plastic that can be autoclaved or washed with pure ethanol or sodium. Features of the Bio-Dot Microfiltration System: Resistance to 100% ethanol, strong acid, and NaOH; Autoclavability; Patented sealing gasket to eliminate lateral leakageMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMouse serum (5 μl) was diluted in 200 μl of 1× PBS and was fixed on a nitrocellulose membrane by suction through Bio-Rad Bio-Dot 96-Well Microfiltration equipment. Features of the Bio-Dot SF Microfiltration System:Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Place a pre-wetted membrane in the 96-well Bio. To examine expression of stress proteins, dot-blot assays were performed in a 96-well plate format using a Bio-dot microfiltration manifold (Bio-Rad, Hercules, CA). For Dot Blotting, lysates containing equivalent amount of total protein were loaded on a 96-well plate (Bio-Dot ® Microfiltration Apparatus; Bio-Rad Laboratories Inc. Leak-free, modular, and autoclavable template. Catalog Number. Bioz Stars score: 96/100, based on 1 PubMed citations. Order Number: 1703938In brief, dot blots of genomic DNA and modified control oligonucleotides (unmethylated: oligo control, 5-methylated CpG: oligo 5mC, and 5-hydroxymethylated CpG: oligo 5hmC) were produced with Bio-Rad’s Bio-Dot Microfiltration apparatus according to manufacturer’s instructions. The Bio-Dot SF has 48 slots and a capacity of 50–500 μl sample. Last modified: July 8, 2003 MATERIALS: DRY Bio-Rad Bio-Dot SF Microfiltration Apparatus Nytran membrane Bio-Dot SF filter paper 60 3mm Whatman paper. 00 (8). Screen and filter antibodies and sera. . Leak-free, modular, and autoclavable template. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. , c a l l 1 - 8 0 0 - 4 B I O R A D ( 1 - 8 0 0 - 4 2 4 - 6 7 2 3 ) Table of Contents. Prezzo di listino: Your Price: Log in to see your price. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Slot Format 96 Well Microfiltration Apparatus, supplied by Bio-Rad, used in various techniques. 12. Bio-Dot ® microfiltration apparatus (Bio-Rad). Table of Contents Page. Screen and filter antibodies and sera. Leak-free, modular, and autoclavable template. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketWe would like to show you a description here but the site won’t allow us. The Bio-Dot microfiltration apparatus can be used for any application requiring rapid immobilization and screening of unfractionated or purified proteins, nucleic acids, or macromolecular complexes on membranes, such as nitrocellulose or Zeta-Probe ® membrane. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. This instrument allows rapid, reproducible applications of up to 96 samples on one membrane sheet. 170-6542. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. Section 3 Dot Blotting. Screen and filter antibodies and sera. Reproducibly capture proteins conversely nucleic acids include solution go membranes with Bio-Dot microfiltration systems. Use the Bio-Dot SF Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 48-well slot format. Leak-free, modular, and autoclavable template. 6 Section 5 Optimization of Blotting. RNA BIO-DOT (‘SLOT BLOT’) Adapted from existing protocols by Vinh Pham. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Leak-free, modular, and autoclavable template. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. ZERO BIAS - scores, article reviews, protocol conditions and moreBio-Dot® Microfiltration Apparatus Instruction Manual Catalog Numbers 170-6545 170-6547 For technical service, call your local Bio-Rad office or, in the U. Leak-free, modular, and autoclavable template. Leak-free, modular, and autoclavable template. 3. leakage, whether the vacuum is on or off. Leak-free, modular, and autoclavable template. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Screen and filter antibodies and sera. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketMicrofiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket Bio-Dot Apparatus #1706545 | Bio-Rad Skip to main contentBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Screen and filter antibodies and sera. Autoclaving 15 minutes at 250°F (121°C) with a 1 minute fast exhaust. Our goal is to support your research with the necessary tools and materials to optimize the analysis of complex protein samples. The gasket is alignedMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketBio-Rad 96 well vacuum dot blot apparatus 96 Well Vacuum Dot Blot Apparatus, supplied by Bio-Rad, used in various techniques. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketConversion of the Bio-Dot apparatus to the Bio-Dot SF apparatus is accomplished by purchasing the Bio-Dot SF module, which provides the 48-well slot format sample template. Leak-free, modular, and autoclavable template. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketMicrofiltration (Dot Blotting) Blotting Systems and Power Supplies 12 Tank Blotting Cells6312 ®Mini Trans-Blot Cell and Criterion ™ Blotter 12 ®Trans-Blot Cell12 Immun-Blot ®Trans-Blot Plus Cell13 Semi-Dry Blotting Cells 13 Trans-Blot® SD Semi-Dry Cell and Detection Kits14 ™Trans-Blot® Turbo System1450 μl of solubilized fractions were loaded on a nitrocellulose membrane (GE Healthcare Life Sciences Amersham) using the Bio-Dot ® 96 wells Microfiltration Apparatus (BIO-RAD). Screen and filter antibodies and sera. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. BIO-RAD Bio-Dot Microfiltratio n Apparatus 84BR 96-well #170-6545 NEW Be the first to write a review. Leak-free, modular, and autoclavable template. The Bio-Dot apparatus is simple to operate. , 1997;. Screen and filter antibodies and sera. The 96-well Bio-Dot and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Leak-free, modular, and autoclavable blueprint. Use the Bio-Dot SF Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 48-well slot format. Screen and filter antibodies and sera. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Leak-free, modular, or autoclavable template. Conversion of the Bio-Dot apparatus to the Bio-Dot SF apparatus is accomplished by purchasing the Bio-Dot SF module, which provides the 48-well slot format sample template. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. ZERO BIAS - scores, article reviews, protocol conditions and moreBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Screen and filter antibodies and sera. To examine expression of stress proteins, dot-blot assays were performed in a 96-well plate format using a Bio-dot microfiltration manifold (Bio-Rad, Hercules, CA). The 96-well Bio-Dot and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. 3 Dot-Blot. 03 for 12. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. Screen and filter antibodies and sera. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Screen and filter antibodies and sera. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Table of Contents Page. Leak-free, modular, and autoclavable template. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketOverview. The Bio-Dot apparatus is simple to operate. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket. Bio-Dot® Microfiltration Apparatus Instruction Manual Catalog Numbers 170-6545 170-6547 For technical service, call your local Bio-Rad office or, in the U. Screen and filter antibodies and sera. Leak-free, modular, and autoclavable template. Leak-free, modular, and autoclavable template. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketPkg of 1, 96-well sample template, modular unit converts Bio-Dot SF apparatus to Bio-Dot apparatus; for blot transfer microfiltrationBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. (a) Large solubilization screening performed in 96-well plate and monitored by dot-blot. Many experimental protocols can be accommodated by using interchangeable templates. The semi. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Touch membrane on dry filter paper to wick off excess. Screen and filter antibodies and sera. Price: US $265. , call 1-800-424-6723. 1 watched in the last 24 hours. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket. Leak-free, modular, and autoclavable template. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Leak-free, modular, and autoclavable template. Sample can be easily applied to the 96-well formate with a standard pipet or with the Costar Octapette pipet. Bio-Dot SF Microfiltration Apparatus,Bio-Rad,#1706542 Casein diet / 3. Screen and filter antibodies and sera. As shown in figure 1, a sheet of membrane is clamped between the gasket and the 96-well sample template. ZERO BIAS - scores, article reviews, protocol conditions and moreMicrofiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketScreening of membrane proteins solubilization. Bio-Dot, from Bio-Rad laboratories, is a simple device that offers a reproducible method for binding proteins or nucleic acids onto nitrocellulose or Zeta-Probe membrane. Leak-free, modular, and autoclavable template. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Many experimental protocols can be accommodated by using interchangeable templates. Bio-Rad Laboratories, Inc. Many experimental protocols can be accommodated by using interchangeable templates. A modified 96-well dot-blot apparatus (Bio-Rad Laboratories Inc. Reproducibly acquire proteins or nucleic sours in solution for membranes with Bio-Dot microfiltration systems. Screen and filter antibodies and sera. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Samples were placed in a 96-well plate with 20 µl 1 M NaOH/25 mM EDTA per well at 95 ℃ for 10 min by Bio-Rad Thermal cycle and put on ice immediately. 75 mm.